Rainfall is a source of water with the best quality in the nature. Therefore, harvesting and using of the runoff resulted from rainfall, can help better management of water resources in our country. As a considerable part of urbanized areas are building roofs, quantity of water harvested from roof catchments will be considerable and can prepare a part of water required for the residents. In this study, rainfall and runoff events were measured in cities of Mashhad (with arid and semi-arid climatic condition) and Noor (with sub-humid and humid climatic condition). The surfaces selected to collect rainwater were two roof catchments (one in each city) with almost the same conditions. After preparing the rooftops, the required storages and the related equipments were established and the amount of runoff was measured after each rainfall event for whole the rainy seasons of the year. Information of precipitation events was received from the closest meteorological synoptic stations. Using appropriate statistical analysis, the volume of runoff as well as runoff coefficient for different levels of rainfall depth and intensity was determined after each event. The results of this research show that average runoff coefficient for the fall season in Mashhad is 0.66 and is 0.75 in Noor, for the winter it is 0.69 and 0.76 respectively in Mashhad and Noor, and for the spring season, the average runoff coefficient was measured 0.62 in Mashhad and 0.69 in Noor. The threshold values (minimum values) of rainfall for runoff generation 0.3 mm in Noor and 0.7 mm in Mashhad. The total depth of rainfall and runoff for the measured period in Mashhad were 217.5 and 139.9 mm, respectively, and in the Noor they were 496.8 and 356.1 mm. The highest amount of seasonal runoff generated in Mashhad belongs to the spring but in Noor it has been occurred in autumn season.